Master Course Description No: BIOE 424 Title: Advanced Systems and Synthetic Biology Credits: 3 UW Course Catalog Description: This course assumes a basic understanding of synthetic and systems biology. It introduces a variety of advanced and more in‐ depth topics on cellular networks, including their operation and engineering. The course is intended for engineering and computer science students. Topics include advanced mathematical modeling of cellular networks; computational standards in systems and synthetic biology; computer algorithms for computational analysis; metabolic flux analysis, control and engineering; protein signaling pathways, analysis, control and engineering. Instructor: Herbert Sauro, Associate Professor, Bioengineering Goals: For students to acquire the necessary tools and knowledge for understanding the dynamic behavior cellular systems together with engineering principles for the redesign of synthetic biochemical systems. Learning Objectives: At the end of this course students will be able to: 1. Understand the different modeling approaches used to represent cellular networks (Structural, Continuous and Stochastic Approaches) 2. Understand the differences between the fundamental cellular subsystems, metabolic, protein and genetic and how this influences potential engineering approaches. 3. Develop an appreciation for the need for standards and ontologies in model exchange and part representation. 4. Understand, implement and use a variety of computational approaching including FBA, MFA, Bifurcation and evolutionary methods. 5. Understand the basic principles of metabolic control including small signal analysis and elementary mode analysis. 6. Learn how to carry out a robustness analysis of a metabolic pathway and propose strategies for engineering pathways. 7. Understand the control of protein networks, highlighting differences and similarities with genetic and metabolic systems. 8. Use computational analysis to study the dynamic properties of protein networks and the design of robust systems. Textbook: U. Alon, Control Systems Engineering, An Introduction to Systems Biology: Design Principles of Biological Circuits, Chapman and Hall, 2006. Reference text: None. Prerequisites by Topic: Introduction to Synthetic Biology, EE 423 T i op cs: 1. The importance of network structure in cellular networks 2. Review of continuous and stochastic models of cellular networks y. 3. The interplay between structure and dynamics onary design approaches in synthetic biolog ML, PoBoL, CAD in synthetic biology) 4. Bifurcation analysis and evoluti 5. Standards and ontologies (SBML, Cell 6. Control systems in metabolism 7. Control systems in protein networks sis of cellular pathways modes, FBA and MFA 8. Robustness and small signal analy 9. Advanced structural analysis including elementary ies dynamical analysis 10. Metabolic engineering strateg 11. Protein networks, control and 12. Protein network engineering Course Structure: The class meets for three lectures a week (MWF). There is weekly homework due; Grading is based on homework, one midterm exam, and a final exam. The grading percentages and nature of the exams are left to the discretion of the instructor. Computer Resources: The course will use MATLAB or any other suitable software platform for homework problems. The students complete an average of 3 hours of computer work per week. Outcome Coverage: (a) An ability to apply knowledge of mathematics, science, and engineering. Lectures and homework deal with the application of differential equations, linear algebra and Laplace transforms to control systems. (c) An ability to communicate effectively. (f) An understanding of biology and physiology. Prepared By: Herbert Sauro Last revised: 4/28/2009